growth factor tgf β  (MedChemExpress)

Journal: Frontiers in Immunology

Article Title: Latilactobacillus curvatus DCF0620 and postbiotics derived from soybean germ reduce colitis severity by modulating fibrosis and gut dysbiosis

doi: 10.3389/fimmu.2025.1726298

Figure Lengend Snippet: L. paracasei DCF0420, L. plantarum DCF0514, and L. curvatus DCF0620 improve inflammation and fibrosis in vitro . (A) Splenocytes from C57BL/6 mice (n = 4-6) were stimulated with each strain separately (at 0.1, 1, or 10 μg/mL) or vehicle (saline) for 2 h and then cultured with anti-CD3 Ab (2 μg/mL) or LPS (500 ng/mL) for 3 days. The levels of IFN-γ, IL-17 and IL-10 in the culture supernatant were determined via ELISA. (B) CCD-18Co cells were treated with each strain separately (at 10 μg/mL) or vehicle (saline) in the presence of TGF-β (10 ng/mL) for 24 h. The levels of fibronectin were assessed via immunoblotting. Values are presented as means ± SDs. Data are representative of two independent experiments.

Article Snippet: The sections were incubated with primary antibodies (Abs) against TNF-α (#ab6671; Abcam, Cambridge, UK), IL-1β (#ab9722; Abcam), IL-6 (#ab7737; Abcam), IL-17 (#ab79056; Abcam), transforming growth factor (TGF)-β (#BS-0086R; Bioss, Woburn, MA, USA), type I collagen (Col1) (#ab6308; Abcam), and α-smooth muscle actin (α-SMA) (#ab7817; Abcam) for 2 h at room temperature.

Techniques: In Vitro, Saline, Cell Culture, Enzyme-linked Immunosorbent Assay, Western Blot

Journal: Frontiers in Immunology

Article Title: Latilactobacillus curvatus DCF0620 and postbiotics derived from soybean germ reduce colitis severity by modulating fibrosis and gut dysbiosis

doi: 10.3389/fimmu.2025.1726298

Figure Lengend Snippet: L. paracasei DCF0420, L. plantarum DCF0514, and L. curvatus DCF0620 reduce colonic fibrosis in mice with colitis. Acute colitis was induced in C57BL/6 mice by oral administration of 2% DSS. Each strain was orally administered to the mice from 7 days before DSS injection until the end of the experiment (n = 4-5/group, normal control n = 3). On day 11, colon tissue sections were stained with Masson’s trichrome and Abs against TGF-β, Col1, and α-SMA. Representative images are shown (original magnification: 400×, Scale bar: 100 µm). Graphs display the numbers of Ab-positive cells per field. Data are expressed as mean ± SDs. Statistical analysis was performed using One-way ANOVA followed by Tukey’s test. *P < 0.05, ****P < 0.0001.

Article Snippet: The sections were incubated with primary antibodies (Abs) against TNF-α (#ab6671; Abcam, Cambridge, UK), IL-1β (#ab9722; Abcam), IL-6 (#ab7737; Abcam), IL-17 (#ab79056; Abcam), transforming growth factor (TGF)-β (#BS-0086R; Bioss, Woburn, MA, USA), type I collagen (Col1) (#ab6308; Abcam), and α-smooth muscle actin (α-SMA) (#ab7817; Abcam) for 2 h at room temperature.

Techniques: Injection, Control, Staining

Journal: Frontiers in Immunology

Article Title: Latilactobacillus curvatus DCF0620 and postbiotics derived from soybean germ reduce colitis severity by modulating fibrosis and gut dysbiosis

doi: 10.3389/fimmu.2025.1726298

Figure Lengend Snippet: Postbiotics from L. curvatus DCF0620 reduce colonic fibrosis and inflammation in mice with colitis. Acute colitis was induced in C57BL/6 mice by oral administration of 2% DSS. Then, 200 μL of postbiotics (100 mg/mL in PBS) was orally administered to the mice from 7 days before DSS injection until the end of the experiment (n = 4-5/group, normal control n = 3). (A) On day 12, colon tissue sections were stained with Abs against TGF-β, Col1, and α-SMA. (B) On day 12, the sections were stained with Abs against TNF-α, IL-1β, IL-6, and IL-17. Representative images are shown (original magnification: 400×, Scale bar: 100 µm). Graphs display the numbers of Ab-positive cells per field. Data are expressed as mean ± SDs. Statistical analysis was performed using One-way ANOVA followed by Tukey’s test. ****P < 0.0001.

Article Snippet: The sections were incubated with primary antibodies (Abs) against TNF-α (#ab6671; Abcam, Cambridge, UK), IL-1β (#ab9722; Abcam), IL-6 (#ab7737; Abcam), IL-17 (#ab79056; Abcam), transforming growth factor (TGF)-β (#BS-0086R; Bioss, Woburn, MA, USA), type I collagen (Col1) (#ab6308; Abcam), and α-smooth muscle actin (α-SMA) (#ab7817; Abcam) for 2 h at room temperature.

Techniques: Injection, Control, Staining

Journal: Journal of Cell Communication and Signaling

Article Title: Regulation of phosphatase and tensin homolog by complement component 5a (C5a) and its receptor (C5aR1) in lupus nephritis: A novel therapeutic target

doi: 10.1002/ccs3.70055

Figure Lengend Snippet: C5aR1A alleviates inflammation and renal dysfunction in LN mice. (A) Schematic diagram of the animal model procedure; (B) mRNA expression levels of IL‐1β and TNF‐α in mouse renal tissue, normalized to β‐actin ( n = 5); (C) PAS and H&E staining of paraffin‐embedded kidney sections ( n = 5); (D, E) Immunohistochemical analysis of IL‐1β, MCP‐1, TNF‐α, and TGF‐β expression in renal tissues at 40× magnification ( n = 5); (F) Western blot analysis of PTEN and p‐AKT in mouse kidney tissue, with GAPDH as a loading control ( n = 5); (G) Quantification of BUN and Scr levels in mouse kidney tissues ( n = 5). Statistical analysis was performed using one‐way ANOVA. p > 0.05, not significant. * p < 0.05; *** p < 0.001; **** p < 0.0001. ANOVA, analysis of variance; BUN, blood urea nitrogen; H&E, hematoxylin and eosin; LN, lupus nephritis; MCP‐1, monocyte chemoattractant protein‐1; PAS, periodic acid–Schiff; PTEN, phosphatase and tensin homolog; Scr, serum creatinine; TGF‐β, transforming growth factor‐β.

Article Snippet: Sections were incubated overnight at 4°C with rabbit primary antibodies, including C5aR1 (Proteintech), IL‐1β (Proteintech, Cat#16806‐1‐AP), monocyte chemoattractant protein‐1 (MCP‐1) (Abcam, Cat#ab25124), TNF‐α (Abcam, Cat#ab6671), and transforming growth factor‐β (TGF‐β) (Proteintech, Cat#21898‐1‐AP).

Techniques: Animal Model, Expressing, Staining, Immunohistochemical staining, Western Blot, Control